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I have just over about 500ml of slurry from an 80 shilling beer(Thats 500 odd ml of compacted slurry). I am wanting to do a wee heavy and use the harvested yeast. It is the Wyeast Scottish Ale yeast and was harvested on the 23rd Dec and been stored in a jar in the fridge since.
Using Mr Malty it says there is only 10% viability left and that i would need 2L yeast slurry for a 1.098 beer at 23L. This seems like an awful lot of yeast to get.
Will my jar of slurry be ok to use or should i start from scratch with new yeast??
If it is ok, what size starter should i use? No stir plate.
Thanks
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Start from scratch. Not only will the viability be extremely low (so you're pitching a ton of dead/autolysing cells into your lovely wort) but you're unlikely to have a pure strain anymore due to the amount of mutants, especially petite mutants, due to starvation.
Dump it, grow some more. :)
You could restreak some of the yeast on an agar plate (if you have the capability), and select a single colony of reasonable size, and propagate.
But it would be a lot easier to start with a new yeast, as Greig suggested.
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